The study investigated influence of health belief on quality of life among key populations accessing care at one stop shop, Kaduna State. The study adopted a cross-sectional survey design using stratified and purposive to 262 participants for the study. The participants comprised 225 were males and 37 were females. Their age ranged from 18 to 50 years with mean age of 28.65 and a standard deviation of 5.78. The instruments used for data collection were: AIDS Health Belief Scale and World Health Organization Quality of Life Brief. Multiple Regression Statistics were used to test the study hypotheses. The results showed that perceived susceptibility at (F4, 257) β= .07, t = 1.23 had no influence on quality of life at p<.05. Perceived severity at (F4, 257) β= -.23*, t = -3.63, and perceived susceptibility at (F4, 257) β= -.27*, t = -4.13 had negative influence on quality of life at p<.05. Perceived benefit at (F4, 257) β= .21*, t = 3.16 had positive influence on quality of life at p<.05. It was concluded that health beliefs significantly influence the quality of life among key populations. Therefore, it was recommended that there should be programs that focus on enhancing health belief information and self-image among individuals.